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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
21/06/2002 |
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Data da última atualização: |
30/03/2017 |
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Autoria: |
WEBER, R. E.; FAGO, A.; VAL, A. L.; BANG, A.; HAUWAERT, M. L. van; DEWILDE, S.; ZAL, F.; MOENS, L. |
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Título: |
Isohemoglobin differentiation in the bimodal-breathing Amazon catfish Hoplosternum littorale. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Journal of Biology Chemistry, v.275, n.23, p.17297-17305, June, 2000. |
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Idioma: |
Inglês |
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Conteúdo: |
The bimodal gill(water)/gut(air)-breeding Amazonian catfish Hoplosternum littorale that frequents hypoxic habitats uses "mammalian" 2,3-diphosphoglycerate (DPG) in addition to
"piscine" ATP and GTP as erythrocytic O2 affinity modulators. Its electrophoretically distinct anodic and cathodic hemoglobins (Hb An and Hb Ca) were isolated for functional and molecular characterization. In contrast to Hb An, phosphate-free Hb Ca exhibits a pronunced reverse Boht effect (increased O2 affinity with decreasing pH) that is obliterated by ATP, and opposite pH dependences of Kt (O2 association constant of low affinity, tense state) and the overall heat of oxygenation. Dose-response curves indicate small chloride effects and pronounced and differentiated phosphate effects, DPG < ATP < GTP < IHP. Hb Ca -O2 equilibria analyzed in terms of the Monod-Wyman-Changeux model show that small T state bond energy differences underlie the differentiated phosphate effects. Synthetic peptides, corresponding to N-terminal fragment of the cytoplasmic domain of trout band 3 protein, undergo oxygenation-linked binding to Hb ca, suggesting a metabolic
regulatory role for this hemoglobin. The amino acid sequences for the chains of Hb Ca obtained by Edman degradation and cDNA sequencing show unusual substitutions at the phosphate-binding site that are discussed in terms of its reserve Bohr effect and anion sensitivities. |
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Palavras-Chave: |
Amazonas; Amazonian Hoplosternum littorale; Bimodal gill; Branquia; Peixe gato; Respiration. |
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Thesagro: |
Respiração. |
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Thesaurus Nal: |
catfish. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02226naa a2200301 a 4500
001 1810129
005 2017-03-30
008 2000 bl --- 0-- u #d
100 1 $aWEBER, R. E.
245 $aIsohemoglobin differentiation in the bimodal-breathing Amazon catfish Hoplosternum littorale.
260 $c2000
520 $aThe bimodal gill(water)/gut(air)-breeding Amazonian catfish Hoplosternum littorale that frequents hypoxic habitats uses "mammalian" 2,3-diphosphoglycerate (DPG) in addition to "piscine" ATP and GTP as erythrocytic O2 affinity modulators. Its electrophoretically distinct anodic and cathodic hemoglobins (Hb An and Hb Ca) were isolated for functional and molecular characterization. In contrast to Hb An, phosphate-free Hb Ca exhibits a pronunced reverse Boht effect (increased O2 affinity with decreasing pH) that is obliterated by ATP, and opposite pH dependences of Kt (O2 association constant of low affinity, tense state) and the overall heat of oxygenation. Dose-response curves indicate small chloride effects and pronounced and differentiated phosphate effects, DPG < ATP < GTP < IHP. Hb Ca -O2 equilibria analyzed in terms of the Monod-Wyman-Changeux model show that small T state bond energy differences underlie the differentiated phosphate effects. Synthetic peptides, corresponding to N-terminal fragment of the cytoplasmic domain of trout band 3 protein, undergo oxygenation-linked binding to Hb ca, suggesting a metabolic regulatory role for this hemoglobin. The amino acid sequences for the chains of Hb Ca obtained by Edman degradation and cDNA sequencing show unusual substitutions at the phosphate-binding site that are discussed in terms of its reserve Bohr effect and anion sensitivities.
650 $acatfish
650 $aRespiração
653 $aAmazonas
653 $aAmazonian Hoplosternum littorale
653 $aBimodal gill
653 $aBranquia
653 $aPeixe gato
653 $aRespiration
700 1 $aFAGO, A.
700 1 $aVAL, A. L.
700 1 $aBANG, A.
700 1 $aHAUWAERT, M. L. van
700 1 $aDEWILDE, S.
700 1 $aZAL, F.
700 1 $aMOENS, L.
773 $tJournal of Biology Chemistry$gv.275, n.23, p.17297-17305, June, 2000.
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